Shmac 5 cell line
SIGMA/10112303 - NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human prostate, Epithelial
Product Type: Chemical
| biological source | human prostate |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Epithelial |
| packaging | pkg of vial of cells 10112303-1VL |
| tube of 5 μg 10112303-DNA-5UG | |
| products | See Lang SH, et.al (2006) |
| receptors | Not specified |
| relevant disease(s) | cancer |
| technique(s) | cell culture | mammalian: suitable |
| Biochem/physiol Actions: | Prostate cancer moderately well differentiated, early stage |
| Biochem/physiol Actions: | STR-PCR Data: Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 11,12 D5S818: 12,14 D7S820: 7,12 THO1: 6,9.3 TPOX: 8,12 vWA: 15,17 |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Packaging: | NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| Preparation Note: | Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day. |
| Preparation Note: | Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |