Shmac 4 cell line
SIGMA/10112302
Product Type: Chemical
| biological source | human prostate |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Epithelial |
| products | See Lang SH, et.al (2006) |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Origin: | Prostate cancer moderately well differentiated, early stage |
| Culture Medium: | Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability. |
| DNA Profile: | STR-PCR Data: Amelogenin: X,Y CSF1PO: 12 D13S317: 11 D16S539: 11,14 D5S818: 12,13 D7S820: 7,10 THO1: 7,9 TPOX: 8 vWA: 17,19 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |