KF1 cell line
SIGMA/10072801
Product Type: Chemical
| biological source | fish fin |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Fibroblast |
| products | Not specified |
| Quality Level | 100  |
| receptors | Not specified |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Description: | The KF1 cell line was developed from Koi carp (Cyprinus carpio koi) fin. |
| Cell Line Origin: | Koi carp fin cell line, koi herpesvirus isolation |
| Culture Medium: | EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). If a CO2 incubator is not available, 10 mM HEPES can be included. |
| DNA Profile: | Not specified |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |