Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, neonatal
SIGMA/100-05N
Product Type: Product-on-demand
Cell Applications : CADMEC/HMVEC monolayer (left), vessel-like structures formed in an angiogenesis assay (middle) and immunolabeled for CD31/PECAM (right, green). Nuclei are visualized with PI (right, red).
| biological source | human neonatal foreskin or adult skin capillaries (normal) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Endothelial |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Endothelial vessel function, cell signaling, angiogenesis |
| Cell Line Origin: | Skin |
| Components: | Cell Basal Medium containing 10% FBS & 10% DMSO |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. CADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence. Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx). |
| Preparation Note: | • 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings |
| Subculture Routine: | Please refer to the CADMEC/HMVEC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |