Ammonium sulfate
SIGMA/09978 - BioUltra, ≥99.0% (T)
Synonym: Mascagnite
CAS Number: 7783-20-2
Empirical Formula (Hill Notation): H8N2O4S
Molecular Weight: 132.14
EC Number: 231-984-1
MDL Number: MFCD00003391
Linear Formula: (NH4)2SO4
Product Type: Chemical
| λ | 1 M in H2O |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| nitrate (NO3-): ≤50 mg/kg | |
| phosphate (PO43-): ≤5 mg/kg | |
| assay | ≥99.0% (T) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.2 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤1 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤2 mg/kg | |
| Fe: ≤2 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤50 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤2 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤1 mg/kg | |
| density | 1.77 g/mL at 25 °C (lit.) |
| form | crystals |
| ign. residue | ≤0.01% (as SO4) |
| impurities | insoluble matter, passes filter test |
| InChI | 1S/2H3N.H2O4S/c;;1-5(2,3) |
| InChI key | BFNBIHQBYMNNAN-UHFFFAOYSA |
| loss | ≤0.1% loss on drying, 110 °C |
| mp | >280 °C (dec.) (lit.) |
| pH | 5.0-6.0 (25 °C, 1 M in H2O) |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | N.N.OS(O)(=O)=O |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: ≤0.037 |
| λ: 280 nm Amax: ≤0.030 |
| Application: | Ammonium sulfate has been used: • to prepare mobile phase A for hydrophobic interaction chromatography (HIC) • as reference material for the thermal decomposition of NH4NO3 (S1–S6) thermal decomposition to N2O (S1-N2O–S6-N2O) • to prepare the stock solution for M63 medium • as a reagent in molecular biology and chromatography • in the precipitation and fractionation of proteins • in the purification of antibodies • for the crystallographic analysis of nucleic acids and proteins • in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography • for Sepharose chromatography of tRNAs using reverse salt gradients • in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis • HPLC analysis of phospholipids |
| Biochem/physiol Actions: | Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char. |
| General description: | Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps. |
| Legal Information: | Sepharose is a registered trademark of Cytiva |
| Other Notes: | The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose® chromatography of tRNAs using reverse salt gradients. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (T) |
| mp | >280 °C (dec.) (lit.) |
| Density | 1.77 g/mL at 25 °C (lit.) |
| UNSPSC | 12352200 |