4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester
SIGMA/09278 - ≥98.0% (HPLC)
Synonym: 4-
CAS Number: 146998-31-4
Empirical Formula (Hill Notation): C19H18N4O4
Molecular Weight: 366.37
MDL Number: MFCD00467415
Linear Formula: C19H18N4O4
Product Type: Chemical
| assay | ≥98.0% (HPLC) |
| fluorescence | λex 428 nm; λem 453 nm |
| form | powder |
| InChI | 1S/C19H18N4O4/c1-22(2)16- |
| InChI key | IBOVDNBDQHYNJI-QZQOTICOSA |
| Quality Level | 100  |
| SMILES string | CN(C)c1ccc(cc1)N=Nc2ccc |
| solubility | DMF: soluble |
| DMSO: soluble | |
| storage temp. | −20°C |
| Analysis Note: | Absorption spectra shows a maximum at 453 nm (in methanol). After reaction with butylamine: max. absorption at 428 nm (Lit.) |
| Application: | A useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies. |
| Other Notes: | N-terminal modification of peptides in automated synthesis |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98.0% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352108 |