MS-1 cell line
SIGMA/09111802
Synonym: MS1 Cells
Product Type: Chemical
| biological source | human skin |
| form | liquid |
| growth mode | Suspension |
| karyotype | Not determined |
| morphology | Loosely packed floating aggregates with irregular outline and no central necrosis |
| products | Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99, |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication) |
| Cell Line Description: | MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (Sigma Catalogue number 09111801). |
| Cell Line Origin: | Human neuroendocrine skin carcinoma |
| Culture Medium: | RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). |
| DNA Profile: | STR-PCR Data: Amelogenin: X CSF1PO: 10 D13S317: 11,14 D16S539: 9 D5S818: 11 D7S820: 8,9 THO1: 7,9.3 TPOX: 8 vWA: 17 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Other Notes: | Depositor and originator: Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213 |
| Subculture Routine: | Seed at 3 x 105 cells/ml in 5% CO2; 37°C Saturation density is approximately 106 cells/ml. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |