MS-1 cell line
SIGMA/09111802 - NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 09111802, human skin, mesothelioma. Loose aggregates with irregular outline, no central necrosis.
Synonym: MS1 Cells
Product Type: Chemical
| biological source | human skin |
| form | liquid |
| growth mode | Suspension |
| karyotype | Not determined |
| morphology | Loosely packed floating aggregates with irregular outline and no central necrosis |
| packaging | pkg of vial of cells 09111802-1VL |
| tube of 5 μg 09111802-DNA-5UG | |
| products | Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99, |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication) |
| Biochem/physiol Actions: | Human neuroendocrine skin carcinoma |
| Biochem/physiol Actions: | MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (Sigma Catalogue number 09111801). |
| Biochem/physiol Actions: | STR-PCR Data: Amelogenin: X CSF1PO: 10 D13S317: 11,14 D16S539: 9 D5S818: 11 D7S820: 8,9 THO1: 7,9.3 TPOX: 8 vWA: 17 |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Other Notes: | Depositor and originator: Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213 |
| Packaging: | NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| Preparation Note: | RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). |
| Preparation Note: | Seed at 3 x 105 cells/ml in 5% CO2; 37°C Saturation density is approximately 106 cells/ml. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |