6-Carboxy-fluorescein diacetate N-succinimidyl ester

SIGMA/08951 - for fluorescence, ≥90.0% (HPLC)

Synonym: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

CAS Number: 150206-15-8
Empirical Formula (Hill Notation): C₂₉H₁₉NO₁₁
Molecular Weight: 557.46
MDL Number: MFCD00269772
Linear Formula: C₂₉H₁₉NO₁₁
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-08951-5MG-F	5 mg	$218.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥90.0% (HPLC)
fluorescence	λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
grade	for fluorescence
InChI	1S/C29H19NO11/c1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
InChI key	JGPOSNWWINVNFV-UHFFFAOYSA-N
Quality Level	100
SMILES string	CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(=O)ON6C(=O)CCC6=O)c1
solubility	DMF: soluble
	DMSO: soluble
storage temp.	−20°C

Application:	The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.
Other Notes:	Reagent for continuously determining the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90.0% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352200

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