U-373 MG (Uppsala) cell line
SIGMA/08061901
Synonym: U-373MG Cells; U373-MG Cells; U373MG Cells
Product Type: Chemical
| biological source | human brain |
| form | liquid |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| morphology | Pleomorphic/astrocytoid |
| Quality Level | 100  |
| receptors | PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991) |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS). |
| Biochem/physiol Actions: | U-373 MG (Uppsala) is a human glioblastoma astrocytoma derived from a malignant tumour by explant technique. |
| Cell Line Description: | A human glioblastoma astrocytoma derived from a malignant tumour by explant technique. This catalogue entry represents a new deposit of U-373 MG obtained from the originator′s laboratory in Uppsala; it has been designated U-373 MG (Uppsala) to distinguish it from U-373 MG (Sigma Catalogue number 89081403) which is now known to be identical, by Short Tandem Repeat (STR)-PCR profiling, to another glioblastoma cell line, U-251 MG (please read below for more information about this discovery). U-373 MG (Uppsala) has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′. |
| Cell Line Origin: | Human glioblastoma astrocytoma |
| Culture Medium: | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS). |
| DNA Profile: | STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 8 D16S539: 12,13 D5S818: 12 D7S820: 8.2,12 THO1: 7,9.3 TPOX: 8 vWA: 17,18 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |