N-Ethylmaleimide
SIGMA/04259 - BioUltra, ≥99.0% (HPLC)
Synonym: NEM
CAS Number: 128-53-0
Empirical Formula (Hill Notation): C6H7NO2
Molecular Weight: 125.13
EC Number: 204-892-4
MDL Number: MFCD00005509
Linear Formula: C6H7NO2
Product Type: Chemical
| absorption | cut-off at 382 nm in methanol at 1 M |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤100 mg/kg | |
| assay | ≥99.0% (HPLC) |
| bp | 210 °C (lit.) |
| cation traces | Al: ≤5 mg/kg |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| ign. residue | ≤0.05% |
| impurities | insoluble matter, passes filter test |
| InChI | 1S/C6H7NO2/c1-2-7-5(8)3-4 |
| InChI key | HDFGOPSGAURCEO-UHFFFAOYSA |
| mp | 43-46 °C (lit.) |
| 44-47 °C | |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | CCN1C(=O)C=CC1=O |
| solubility | methanol: 1 M at 20 °C, clear, colorless |
| storage temp. | 2-8°C |
| Application: | N-Ethylmaleimide (NEM) has been used: • to prepare NEM-modified myosin beads • to block unmodified free thiols in samples • as a supplement in IP lysis/wash buffer for in vitro SUMOylation assay |
| Application: | Reagent for the covalent modification of cysteine residues in proteins. |
| Biochem/physiol Actions: | Augments currents from native M-channels in sympathetic neurons and acts as an opener for KCNQ2, KCNQ4 and KCNQ5 channels. |
| Biochem/physiol Actions: | Sulfhydryl alkylating agent that inactivates NADP-dependent isocitrate dehydrogenase and many endonucleases. |
| Symbol |   GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H300 - H311 - H314 - H317 |
| Precautionary statements | P260 - P280 - P301 + P310 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310 |
| Hazard Codes | T+ |
| Risk Statements | 21-28-34-43 |
| Safety Statements | 26-28-36/37/39-45 |
| RIDADR | UN 2928 8(6.1) / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 163.2 °F |
| Flash Point(C) | 72.9 °C |
| Purity | ≥99.0% (HPLC) |
| bp | 210 °C (lit.) |
| mp | 43-46 °C (lit.); 44-47 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352106 |