Methylene Blue solution
SIGMA/03978 - for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution
Synonym: Methylene blue stain; Ehrlich’s reagent III; Loeffler’s Methylene Blue
CAS Number: 61-73-4
Empirical Formula (Hill Notation): C16H18ClN3S
Molecular Weight: 319.85
MDL Number: MFCD00012111
Linear Formula: C16H18ClN3S
Product Type: Chemical
| εmax | 9.4 at 656-666 nm in water |
| application(s) | diagnostic assay manufacturing hematology histology |
| concentration | 1.5% (1.5 g/ 100 mL) |
| density | 1.0 g/mL at 20 °C |
| form | liquid |
| grade | for microscopy |
| InChI | 1S/C16H18N3S.ClH/c1-18(2) |
| InChI key | CXKWCBBOMKCUKX-UHFFFAOYSA |
| quality | concentrated, aqueous solution |
| Quality Level | 100  |
| shelf life | ~3 yr |
| SMILES string | [Cl-].CN(C)c1ccc2N=C3C=C |
| storage temp. | room temp |
| Application: | Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes. |
| Application: | Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria. |
| General description: | Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright’s stain and Jenner’s stain. |
| General description: | Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.0 g/mL at 20 °C |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |