MDCK-II Cell Line canine
SIGMA/00062107 - NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Synonym: MDCK Type II Cells; MDCK-2 Cells; MDCKII-WT Cells
Product Type: Chemical
| biological source | canine kidney |
| description | Canine Cocker Spaniel Kidney |
| form | liquid |
| growth mode | Adherent |
| karyotype | Dog |
| morphology | Polarised epithelial |
| packaging | pkg of vial of cells 00062107-1VL |
| tube of 5 μg 00062107-DNA-5UG | |
| products | Not specified |
| Quality Level | 100  |
| receptors | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| Biochem/physiol Actions: | Canine Cocker Spaniel Kidney |
| Biochem/physiol Actions: | MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Packaging: | NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| Preparation Note: | EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442) |
| Preparation Note: | Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C, |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |