Chloroform
SIGALD/650498 - HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Synonym: Methylidyne trichloride; Trichloromethane
CAS Number: 67-66-3
Empirical Formula (Hill Notation): CHCl3
Molecular Weight: 119.38
EC Number: 200-663-8
MDL Number: MFCD00000826
Linear Formula: CHCl3
Product Type: Chemical
| λ | H2O reference |
| application(s) | food and beverages |
| assay | ≥99.9% |
| bp | 60.5-61.5 °C (lit.) |
| contains | amylenes as stabilizer |
| density | 1.48 g/mL at 25 °C |
| 1.492 g/mL at 25 °C (lit.) | |
| evapn. residue | ≤0.0001% |
| form | liquid |
| grade | HPLC Plus |
| halogenated residue | <10 ng/L (as heptachlor epoxide) |
| impurities | ≤0.02% water |
| ≤0.5 ppb Fluorescence (quinine) at 365 nm | |
| InChI | 1S/CHCl3/c2-1(3)4/h1H |
| InChI key | HEDRZPFGACZZDS-UHFFFAOYSA |
| mp | −63 °C (lit.) |
| Quality Level | 100  |
| refractive index | n |
| SMILES string | ClC(Cl)Cl |
| suitability | suitable for residue analysis |
| technique(s) | gas chromatography (GC): suitable |
| HPLC: suitable | |
| UV absorption | λ: 245 nm Amax: 1.00 |
| λ: 250 nm Amax: 0.30 | |
| λ: 275-400 nm Amax: 0.005 | |
| vapor density | 4.1 (vs air) |
| vapor pressure | 160 mmHg ( 20 °C) |
| Application: | Chloroform may be used in the following: • Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods • Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD) • Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabi • Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification • Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS • Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry |
| Application: | Chloroform was used in the making of silanized coverslips for DNA combing. It may be used as: • Extractant in solvent extraction process. • A component of the binary mobile phases for liquid chromatography–atmospheri • Solvent for recrystallization. |
| Application: | Suitable for HPLC, spectrophotometry, environmental testing |
| General description: | Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent. |
| Other Notes: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Packaging: | 1, 6×1, 4, 4×4 L in glass bottle |
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| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 |
| Precautionary statements | P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 63-20-22-36/38-40-48/20/22-67 |
| Safety Statements | 26-36/37 |
| RIDADR | UN 1888 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥99.9% |
| bp | 60.5-61.5 °C (lit.) |
| mp | −63 °C (lit.) |
| Density | 1.48 g/mL at 25 °C; 1.492 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12190000 |