Formamide
SIGALD/47670 - puriss. p.a., ACS reagent, ≥99.5% (GC/T)
Synonym: Amide C1; Formic amide
CAS Number: 75-12-7
Empirical Formula (Hill Notation): CH3NO
Molecular Weight: 45.04
EC Number: 200-842-0
MDL Number: MFCD00007941
Linear Formula: HCONH2
Product Type: Chemical
assay | ≥99.5% (GC/T) |
autoignition temp. | 932 °F |
bp | 210 °C (lit.) |
cation traces | Ca: ≤5 mg/kg |
Cd: ≤1 mg/kg | |
Co: ≤1 mg/kg | |
Cr: ≤1 mg/kg | |
Cu: ≤1 mg/kg | |
Fe: ≤1 mg/kg | |
K: ≤20 mg/kg | |
Mg: ≤1 mg/kg | |
Mn: ≤1 mg/kg | |
Na: ≤20 mg/kg | |
Ni: ≤1 mg/kg | |
Pb: ≤1 mg/kg | |
Zn: ≤1 mg/kg | |
density | 1.132 g/mL at 20 °C |
1.134 g/mL at 25 °C (lit.) | |
expl. lim. | 2.7-19 % |
form | liquid |
grade | ACS reagent |
puriss. p.a. | |
impurities | ≤0.02% free acid (as HCOOH) |
≤0.1% water | |
InChI | 1S/CH3NO/c2-1-3/h1H,(H2,2 |
InChI key | ZHNUHDYFZUAESO-UHFFFAOYSA |
mp | 2-3 °C (lit.) |
pH | 4-10 (20 °C, 200 g/L) |
Quality Level | 200 |
refractive index | n |
n |
|
SMILES string | NC=O |
suitability | in accordance for miscibility |
technique(s) | PCR: suitable |
transition temp | transition temp 2.0-3.0 °C |
vapor density | 1.55 (vs air) |
vapor pressure | 0.08 mmHg ( 20 °C) |
30 mmHg ( 129 °C) |
Application: | Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures. |
General description: | Formamide is a colorless organic solvent. It has been reported to increase the precision of PCR (Polymerase Chain Reaction) studies. Microwave spectral study of its isotopic forms suggests that the molecule of formamide is nonplanar in which the H2N-C group exhibits a shallow pyramid like structure. |
Packaging: | 1, 2.5 L in glass bottle |
Packaging: | 4×25, 250 mL in glass bottle |
Symbol | GHS08 |
Signal word | Danger |
Hazard statements | H351 - H360D - H373 |
Precautionary statements | P201 - P202 - P260 - P280 - P308 + P313 - P405 |
Hazard Codes | T |
Risk Statements | 61-40-48/22 |
Safety Statements | 53-36/37-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | 305.6 °F |
Flash Point(C) | 152 °C |
Purity | ≥99.5% (GC/T) |
bp | 210 °C (lit.) |
mp | 2-3 °C (lit.) |
Density | 1.132 g/mL at 20 °C; 1.134 g/mL at 25 °C (lit.) |
Refractive Index | n |
UNSPSC | 12352111 |