Nitrotetrazolium Blue chloride

SIAL/N6639 - powder, electrophoresis grade

Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride; 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT

CAS Number: 298-83-9
Empirical Formula (Hill Notation): C₄₀H₃₀Cl₂N₁₀O₆
Molecular Weight: 817.64
EC Number: 206-067-4
MDL Number: MFCD00012159
Linear Formula: C₄₀H₃₀N₁₀O₆ · 2Cl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-N6639-50MG	50 mg	$52.90 1/EA	Add To Favorites
45-N6639-250MG	250 mg	$125.00 1/EA	Add To Favorites
45-N6639-1G	1 g	$195.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N6639-250MG.

Properties
Descriptions
Safety Info.
Basic Data

assay	>90%
form	powder
grade	electrophoresis grade
InChI	1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key	FSVCQIDHPKZJSO-UHFFFAOYSA-L
Quality Level	100
SMILES string	[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
solubility	2-methoxyethanol: 20 mg/mL
	ethanol: 5 mg/mL
	H₂O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.)
storage temp.	2-8°C
technique(s)	electrophoresis: suitable

Application:	Nitrotetrazolium Blue chloride has been used: • to determine the superoxide anion in skeletal muscle cells • as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections • as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O₂- ) anion in Brassica juncea seedlings • as a component in Triton X-100 solution to perform staining of coronal brain sections
Biochem/physiol Actions:	Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O₂.
General description:	Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
Packaging:	1 g in glass bottle
Packaging:	50, 250 mg in glass insert

Symbol	GHS02
Signal word	Danger
Hazard statements	H242
Precautionary statements	P210 - P234 - P235 - P240 - P370 + P378 - P403
Hazard Codes	Xn
Risk Statements	22
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Purity	>90%
Storage Temp.	2-8°C
UNSPSC	12171500

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