Fast Blue B Salt
SIAL/D9805 - Dye content ~95 %
Synonym: o-Dianisidine bis(diazotized) zinc double salt; Azoic Diazo No. 48; DBB; Diazo Blue B; Naphthanil Diazo Blue B
CAS Number: 14263-94-6
Empirical Formula (Hill Notation): C14H12Cl4N4O2Zn
Molecular Weight: 475.47
EC Number: 238-153-2
MDL Number: MFCD00075203
Linear Formula: C14H12N4O2Cl2 · ZnCl2
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| color | yellow to dark brown |
| composition | Dye content, ~95% |
| description | Technical grade |
| form | powder |
| InChI | 1S/C14H12N4O2.4ClH.Zn/c1- |
| InChI key | GPPKNJIWDULNQH-UHFFFAOYSA |
| mp | >300 °C (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | [Cl-].[Cl-].Cl[Zn]Cl.COc1 |
| solubility | H2O: 1 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | microbe id | staining: suitable |
| Application: | • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens. • It has been used as a spraying agent in thin layer chromatography for the identification of separated components. • It has also been used for the determination of the lipase activity. |
| Biochem/physiol Actions: | Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity. |
| Packaging: | 10, 100 g in glass bottle |
| Symbol | ![]() GHS02,GHS08 |
| Signal word | Danger |
| Hazard statements | H242 - H350 |
| Precautionary statements | P202 - P210 - P235 - P308 + P313 - P370 + P378 - P403 |
| Hazard Codes | T |
| Risk Statements | 45 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | >300 °C (lit.) |
| Storage Temp. | 2-8°C |
| Colour Index Number | 37235 |
| UNSPSC | 12171500 |



