Protein Standard Mix 15 - 600 kDa
SIAL/69385 - for size exclusion chromatography
Product Type: Chemical
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| analyte chemical class(es) | amino acids, peptides, proteins |
| application(s) | clinical food and beverages pharmaceutical |
| form | solid |
| format | neat |
| mol wt | 15-600 kDa |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | gel permeation chromatography (GPC): suitable |
| Application: | This analytical standard is used for the following:• Evaluation of selectivity and separation efficiency of size exclusion chromatography (SEC) to separate intact proteins by varying flow rate, size of silica particles and pore sizes in the column • Simultaneous determination of oligomerized and nitrated proteins by size exclusion chromatography-high performance liquid chromatography-diode array detection (SEC-HPLC-DAD) • Molecular weight separation of proteins by size-exclusion chromatography, formed upon O3 and NO2 induced oxidation, nitration, and oligomerization of bovine serum albumin (BSA) as a model protein • Estimation of molecular masses of two recombinant proteins— TNF fluorescent sensor (BTN-Kat) and fluorescent sensor-inhibitor (ITN-Kat), by size exclusion chromatography (SEC) to evaluate their ability of binding and neutralizing tumor necrosis factor (TNF) in vitro and further serving as imaging labels for non-invasive analysis |
| General description: | The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa. Components Thyroglobulin bovine MW ~ 670 000 Da γ-globulins from bovine blood MW ~ 150 000 Da Ovalbumin MW~ 44 300 Da Ribonuclease A type I-A MW ~ 13 700 Da p-aminobenzoic acid (pABA) MW ~ 137 Da |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 + H312 + H332 - H334 - H412 |
| Precautionary statements | P261 - P264 - P273 - P280 - P301 + P312 - P302 + P352 + P312 |
| Hazard Codes | T |
| Risk Statements | 24/25-52/53 |
| Safety Statements | 36/37-45-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 85151701 |