Lipase A Candida antarctica, recombinant from Aspergillus oryzae
SIAL/62287 - powder, beige, ~2 U/mg
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
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| color | beige |
| form | powder |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| Quality Level | 100  |
| recombinant | expressed in Aspergillus oryzae |
| shelf life | limited shelf life, expiry date on the label |
| specific activity | ~2 U/mg |
| storage temp. | 2-8°C |
| Application: | Lipase A Candida antarctica, recombinant from Aspergillus oryzae has been used in the enzyme-catalyzed hydrolysis studies with 3-acetylthiohexanal. It has also been used as a biocatalyst to test its effect on the esterification of phytosterol with caprylic acid. |
| Biochem/physiol Actions: | Lipase A Candida antarctica (CALA) is a thermostable serine hydrolase with a higher sn-2 preference towards triglycerides. It displays excellent activity towards large substrates as well as bulky sterically hindered tertiary alcohols. |
| Biochem/physiol Actions: | Lipase A from Candida Antarctica catalyzes hydrolysis of tertiary alcohols to form glycerol and fatty acids and shows selectivity for the N-acylation of β-amino esters. |
| General description: | Lipase A Candida antarctica (CALA) belongs to the α/β hydrolase family. The catalytic triad comprises Ser184, His366, and Asp334 residues. |
| Other Notes: | 1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314, as substrate); 1 U as described above is equivalent to ~0.15 U using trybutyrine, Cat. No. 91010, as substrate, at pH 8.0 and 70°C |
| Other Notes: | Characterization |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~2 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |