Super-DHB

SIAL/50862 - suitable for matrix substance for MALDI-MS, ≥99.0%

CAS Number: 63542-76-7
MDL Number: MFCD08705395
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-50862-10X10MG-F	10 mg	$0.00 10/EA CALL FOR PRICE	Add To Favorites
45-50862-1G-F	1 g	$109.00 1/EA	Add To Favorites
45-50862-5G-F	5 g	$0.00 1/EA CALL FOR PRICE	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

analyte chemical class(es)	glycans, peptides, proteins
analyte functional class(es)	polymers
assay	≥99.0%
	≥99.0% (sum of DHB and 2-hydroxy-5-methoxybenzoic acid, HPLC)
cation traces	Ca: ≤5 mg/kg
	Cd: ≤5 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤5 mg/kg
	Fe: ≤5 mg/kg
	K: ≤5 mg/kg
	Li: ≤5 mg/kg
	Mg: ≤5 mg/kg
	Mn: ≤5 mg/kg
	Na: ≤5 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤5 mg/kg
	Zn: ≤5 mg/kg
Quality Level	100
solubility	methanol: 1%, clear
suitability	suitable for matrix substance for MALDI-MS
technique(s)	MALDI-MS: suitable

Analysis Note:	9:1 mixture of DHB and 2-hydroxy-5-methoxybenzoic acid
Application:	Glycan and Protein Analysis of Glycoengineered Bacterial E. coli Vaccines by MALDI-in-Source Decay FT-ICR Mass Spectrometry: This study demonstrates the application of Super-DHB as a matrix in advanced mass spectrometry for the detailed characterization of glycoengineered vaccines, essential for researchers in pharmaceuticals and life science manufacturing (Nicolardi et al., 2022 ). An Improved Method for Rapid Detection of Mycobacterium abscessus Complex Based on Species-Specific Lipid Fingerprint by Routine MALDI-TOF: Highlights the use of Super-DHB in enhancing the lipid fingerprinting capabilities of MALDI-TOF mass spectrometry, critical for rapid microbial identification in medical microbiology and pharmaceutical research (Jia Khor et al., 2021 ). Discrimination of bovine milk from non-dairy milk by lipids fingerprinting using routine matrix-assisted laser desorption ionization mass spectrometry: Illustrates the role of Super-DHB in differentiating dairy and non-dairy milks based on lipid profiles using MALDI-MS, vital for food scientists and chemists in quality control and food safety (England et al., 2020 ).
General description:	The “super-DHB” matrix consists of a 9:1 (w/w) mixture of 2,5-DHB and 2-hydroxy-5-methoxybenzoic acid, respectively, since this ratio was found to be the most effective. The beneficial effect of the 2-hydroxy- 5-methoxybenzoic acid additive is attributed to a disorder in the 2,5-DHB crystal lattice resulting in enhanced ion desorption (i.e. ‘softer’ desorption) and reduced internal energy, which in turn results in reduced metastable fragmentation. An increase in sensitivity of up to 2 to 3-fold has been reported for a standard dextran mixture with concomitant enhanced resolution attributed to reduced metastable ion formation. The super-DHB matrix has also been reported to generate enhanced signals from tryptic glycopeptides. The use of super-DHB matrix is reported to reduce the presence of matrix background ions and result in glycan signals exhibiting better signal to noise ratios and higher resolution in the mass spectrum.
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Hazard Codes	Xn
Risk Statements	22-36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (sum of DHB and 2-hydroxy-5-methoxybenzoic acid, HPLC); ≥99.0%
UNSPSC	23151816

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