Bile esculin agar
SIAL/48300 - suitable for microbiology, NutriSelect® Plus
Synonym: B.B.E. Agar; Bacteroides Bile Esculin Agar; Esculin Bile Agar
Product Type: Chemical
Selective medium used for isolation and presumptive identification of faecal Streptococci.
| agency | according to ISO 10273:2017 |
| application(s) | environmental food and beverages water monitoring |
| microbiology | |
| composition | agar, 15.0 g/L |
| esculin, 1 g/L | |
| ferric citrate, 0.5 g/L | |
| meat extract, 3 g/L | |
| meat peptone, 5 g/L | |
| ox-bile, 40 g/L | |
| final pH | 6.6±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | Streptococcus spp. |
| selective and differential for Enterococcus spp. | |
| technique(s) | microbe id | specific enzyme detection: suitable |
| Application: | Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism’s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase. |
| Application: | For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970). |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |