N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
SIAL/03450 - purum, ≥98.0% (AT)
Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC; EDC hydrochloride; WSC hydrochloride
CAS Number: 25952-53-8
Empirical Formula (Hill Notation): C8H17N3 · HCl
Molecular Weight: 191.70
EC Number: 247-361-2
MDL Number: MFCD00012503
Linear Formula: C8H17N3 · HCl
Product Type: Chemical
assay | ≥98.0% (AT) |
form | powder |
grade | purum |
InChI | 1S/C8H17N3.ClH/c1-4-9-8-1 |
InChI key | FPQQSJJWHUJYPU-UHFFFAOYSA |
mp | 110-115 °C |
110-115 °C (lit.) | |
Quality Level | 200 |
SMILES string | Cl.CCN=C=NCCCN(C)C |
solubility | H2O: soluble 1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow |
storage temp. | −20°C |
Application: | N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used modify the cell surface of Escherichia coli to covalently couple substances. It has also been used as an activator to modify microfluidic chips to capture Escherichia coli. |
Biochem/physiol Actions: | N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates. |
Biochem/physiol Actions: | Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided. |
Symbol | GHS06,GHS08,GHS09 |
Signal word | Danger |
Hazard statements | H302 - H311 - H315 - H317 - H373 - H410 |
Precautionary statements | P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314 |
Hazard Codes | Xi |
Risk Statements | 37/38-41 |
Safety Statements | 26-36/37/39 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98.0% (AT) |
mp | 110-115 °C (lit.); 110-115 °C |
Storage Temp. | −20°C |
UNSPSC | 12352111 |