X-tremeGENE™ HP DNA Transfection Reagent
ROCHE/XTGHP-RO - High-performance polymer reagent for transfecting many cell lines
Product Type: Chemical
X-tremeGENE HP DNA Transfection Reagent
An effective balance of high efficiency and low cytotoxicity. CHO-K1 cells were transfected with a GFP expression plasmid, with two different transfection reagents using reagent/DNA ratios recommended by each of the suppliers. Panels A and C show the fluorescent signals after transfection. Panels B and D are the corresponding bright field photomicrographs. After transfection with LTX P, fluorescent signals are visible, but there are fewer viable cells compared to transfection with X-tremeGENE HP Transfection Reagent. Cell imaging and analysis was performed using the Roche Cellavista System.
X-tremeGENE HP DNA Transfection Reagent outperforms the competitor reagent. Four hard-to-transfect cell lines were transfected in serum-containing medium using X-tremeGENE HP Reagent. Transfection efficiency was measured as a ratio of GFP-transfected cells versus whole cell number using the Cellavista System and normalized to the reference reagent set at 1. Error bars show the standard deviation of the mean of triplicates.
Performance comparison with competitor reagents. Comparison using a luciferase encoding plasmid in HeLa cells with transfection reagent to DNA ratios as recommended by the respective suppliers is shown. Error bars show the standard deviation of the mean of triplicates.
Cell survival comparison with competitor reagents. HeLa cells were transfected with X-tremeGENE HP Transfection Reagent and two different competitor reagents. Reagent cytotoxicity was measured using the Roche LDH Cytotoxicity Detection Kit (LDH). The applied ratios of microliter reagent to microgram DNA are indicated. X-tremeGENE HP DNA Transfection Reagent showed the lowest levels of cytotoxicity.
| form | liquid (aqueous solution) |
| grade | Molecular Biology |
| manufacturer/tradename | Roche |
| packaging | pkg of 0.4 mL (06366244001) |
| pkg of 1.0 mL (06366236001) | |
| pkg of 5 × 1 mL (06366546001) | |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | transfection: suitable |
| usage | 1 mL (suitable for 165 transfections) |
| Analysis Note: | Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification. |
| Features and Benefits: | X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116). • Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium. • Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents. • Generate physiologically relevant data using a reagent with low cytotoxic effects. • Increase experimental throughput and enable target evaluation using a simple and consistent protocol. |
| General description: | X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells. |
| Legal Information: | X-tremeGENE is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates. |
| Symbol |   GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 |
| Precautionary statements | P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 |
| RIDADR | UN1170 - class 3 - PG 2 - Ethanol, solution |
| WGK Germany | WGK 1 |
| Flash Point(F) | 334.4 °F |
| Flash Point(C) | 168 °C |
| Storage Temp. | −20°C |
| UNSPSC | 41106502 |