X-tremeGENE™ 360 Transfection Reagent
ROCHE/XTG360-RO - Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
Synonym: transfection reagent
Product Type: Chemical
form | liquid (aqueous solution) |
grade | for molecular biology |
manufacturer/tradename | Roche |
packaging | pkg of 0.4 mL (08724105001) |
pkg of 1.0 mL (08724121001) | |
pkg of 5 × 1.0 mL (08724156001) | |
storage temp. | −20°C |
technique(s) | transfection: suitable |
Application: | X-tremeGENE™ 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components. • Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2. • Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools. • Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex. • Suitable for transient and stable transfection. • Functions well in the presence or absence of serum. |
Features and Benefits: | X-tremeGENE™ 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE™ 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE™ 360 exhibits: • Peak performance in a broad range of cell types • Suitable for multiple applications • Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components |
General description: | X-tremeGENE™ 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE™ 360 delivers high efficiency transfection in more cell types with low cytotoxicity. |
Legal Information: | X-tremeGENE is a trademark of Roche |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Physical form: | Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE™ 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates. |
Quality: | Each lot of X-tremeGENE™ 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification. |
Symbol | GHS02,GHS07 |
Signal word | Danger |
Hazard statements | H225 - H319 |
Precautionary statements | P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 - P403 + P235 - P501 |
WGK Germany | WGK 1 |
Flash Point(F) | 61.7 °F |
Flash Point(C) | 16.5 °C |
Storage Temp. | −20°C |
UNSPSC | 41106502 |