Trypsin Sequencing Grade, modified
ROCHE/TRYPSEQM-RO - from bovine pancreas
Synonym: Trypsin
Product Type: Chemical
| form | lyophilized (salt-free) |
| manufacturer/tradename | Roche |
| optimum pH | 8.0 |
| packaging | pkg of 4 × 100 μg (11418033001) |
| pkg of 4 × 25 μg (11418025001) | |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins. It is used for: • Protein-structure elucidation • Tryptic mapping • Fingerprinting analysis • Sequence analysis • Translocation studies • Protein identification |
| General description: | Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified. Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antit |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 1/100 to 1/5 of the protein by weight Storage conditions (working solution): -15 to -25 °C Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations. A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity. |
| Quality: | Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC. |
| Specificity: | Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |