Taq DNA Polymerase (1 U/μl), dNTPack
ROCHE/TAQNL-RO - suitable for PCR, optimum pH ~9.0 (20 °C)
Synonym: Taq | PCR | DNA amplification
Product Type: Chemical
| feature | dNTPs included |
| hotstart: no | |
| input | purified DNA |
| manufacturer/tradename | Roche |
| optimum pH | ~9.0 (20 °C) |
| packaging | pkg of 1,000 U (04738241001 [4 x 250 U]) |
| pkg of 250 U (04738225001) | |
| parameter | 72 °C optimum reaction temp. |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for ≤2,000 reactions (04738241001) |
| sufficient for ≤500 reactions (04738225001) |
| Application: | Taq DNA Polymerase (1 U/μl), dNTPack may be used for: • PCR • RT-PCR • Other primer-extension reactions, such as sequencing and labeling |
| General description: | Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 5 components |
| Unit Definition: | One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetripho Unit Assay: Incubation buffer: 67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP. Incubation procedure: M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation. Volume Activity: 1 U/μl |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 41106300 |
| Components | Taq DNA Polymerase; PCR Buffer with MgCl<sub>2</sub> 10x concentrated; MgCl<sub>2</sub> Stock Solution; PCR Buffer without MgCl<sub>2</sub>; PCR Nucleotide Mix</ul> |