T4 DNA Polymerase
ROCHE/T4DNAPOL-RO - from T4 plasmid pTL43W infected Escherichia coli 71–18
Synonym: DNA polymerase; T4; polymerase
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 U (11004786001) |
| pkg of 500 U (11004794001) | |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| Application: | • Sample Materials: DNA with 5′-protruding ends Site-directed mutagenesis: Together with T4 gene 32 protein, T4 DNA polymerase can fill in single-stranded gaps during site-directed mutagenesis experiments. • Superiority of hybridization probes prepared with T4 DNA polymerase: Polymerase-labeled probes have two advantages over probes prepared by nick translation: a) They do not have artificial hairpin structures. b) They can easily be converted into strand-specific probes by restriction endonuclease cleavage. |
| Application: | T4 DNA polymerase is used to label 3′-termini of DNA. Extensive labeling is achieved by the replacement reaction, in which the 3′-exonuclease activity of the enzyme first digests dsDNA to produce molecules with recessed 3′-termini. On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3′ ends along the length of the template. Exonuclease III from E. coli can be used to create partially single-stranded dsDNA for subsequent polymerization reactions. Molecules labeled to high specific activity are used primarily as hybridization probes. They have two advantages over probes prepared by nick translation: they lack artificial hairpin structures and they can easily be converted into strand-specific probes by cleavage with suitable restriction endonucleases. In combination with T4 Gene 32 Protein, T4 DNA polymerase is used for gap-filling in site-directed mutagenesis experiments. |
| General description: | T4 DNA Polymerase is a DNA-dependent DNA polymerase that catalyzes the addition of deoxynucleoside-5′-tripho The enzyme has a very active 3′→5′ exonuclease with a high specificity for single-stranded DNA; it lacks 5′→3′ exonuclease activity. |
| General description: | T4 DNA Polymerase is a DNA-dependent DNA polymerase that catalyzes the addition of deoxynucleoside-5′-tripho The enzyme has a very active 3′→5′ exonuclease with a high specificity for single-stranded DNA; it lacks 5′→3′ exonuclease activity. Contents • Enzyme Solution, 1 U/μl, in buffer, pH 8.0 • Incubation Buffer, 5x concentrated |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 2 components |
| Quality: | Absence of endonucleases, ss-specific DNases, nicking activity, and RNases tested according to the current Quality Control procedures. |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |
| Components | Enzyme Solution, in buffer, pH 8.0 1 U/μl; Incubation Buffer 5x concentrated |