Streptavidin, recombinant
ROCHE/RSTREP-RO - lyophilized, ≥17 units/mg protein, pH range 5.0-9.0
Synonym: streptavidin
Product Type: Chemical
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | ~52 kDa |
| packaging | pkg of 1 mg (11721666001) |
| pkg of 5 mg (11721674001) | |
| pH range | 5.0-9.0 |
| pI | 7.4-7.7 (two main bands) |
| Quality Level | 100  |
| solubility | water: 10 mg/L (mA405=100) |
| specific activity | ≥17 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1) KD: 10-15M |
| Reconstitution: | Add 1 ml double-distilled water to get a final concentration of 1 mg/ml (11 721 666 001) or 5 mg/ml (11 721 674 001), respectively. |
| Sequence: | Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site. Each subunit has six tyrosine residues.The protein is free of carbohydrate. |
| Unit Definition: | One unit binds 1 μg D-biotin at pH 8.9. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ≥17 units/mg protein |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |