Proteinase K, recombinant, PCR Grade
ROCHE/RPROTKSOL-RO - Solution from Pichia pastoris
Product Type: Chemical
Proteinase K, recombinant, PCR Grade
| form | buffered aqueous solution (18 ± mg/mL; pH 7.5) |
| manufacturer/tradename | Roche |
| optimum pH | 6.5 and 9.5 |
| packaging | pkg of 1.25 mL (03115887001) |
| pkg of 25 mL (03115844001) | |
| pkg of 5 mL (03115828001) | |
| pH range | 4.0-12.5 |
| Quality Level | 100  |
| recombinant | expressed in Pichia pastoris |
| specific activity | ~2.5 units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | This preparation is free of RNases, DNases, and DNA, according to the current quality control procedures. Absence of Nucleases: Each lot is tested on various substrates to ensure the absence of endonuclease, exonuclease, ribonuclease, and nicking activity. DNA Content: ≤10pg/mg enzyme (determined by Threshold) Bioburden: ≤5cfu/g (determined by the most stringent test from the European Pharmacopoeia, which identifies the total number of viable aerobic bacteria, yeast, and fungi) |
| Application: | • This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines. • The enzyme promotes cell lysis by activating a bacterial autolytic factor. • Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces. • The enzyme is particularly well suited for isolating nucleic acids for amplification reactions. • Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization. |
| Features and Benefits: | Proteinase K cleaves proteins between amino acids X and Y (X-↓-Y), when X = an aliphatic, aromatic, or hydrophobic amino acid, and Y = any amino acid. The enzyme is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. • Choose an effective tool for template preparation. Inactivate DNases and RNases of most species. • Count on consistent quality and performance. Stringent quality testing ensures optimal stability and high-level lot-to-lot performance. • Prepare samples over a wide range of conditions. The robust enzyme is stable over a wide pH range and is ideal for diverse applications. • Benefit from a contamination-free enzyme. The enzyme is tested for the absence of RNases and DNases, and is virtually free of DNA. It is especially suited for the isolation of PCR templates. |
| General description: | Approximately 2.5 U/mg (Chromozym assay); ≥30 U/mg hemoglobin assay. Refer to the Certificate of Analysis for specific values for the present lot. |
| Legal Information: | Pefabloc is a registered trademark of Pentapharm |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | For the rapid inactivation of RNases and DNases. The enzyme can reduce protein to free amino acids if it is present in large excess for long incubation periods. |
| Other Notes: | Volume Activity: Approximately 50 U/ml solution (chromozyme assay); approximately 600 U/ml solution (hemoglobin assay). One unit is the enzyme activity which cleaves at +25 °C in 1 min 18 mmol Chromozym TRY (equivalent to 600 U/ml with the hemoglobin assay). Refer to the Certificate of Analysis for specific values for the present lot. |
| Preparation Note: | Activator: To stimulate proteinase K activity, denaturing agents (SDS and urea) can be added. For example, SDS at a final concentration of 2% can increase the activity of proteinase K significantly. Optimization using denaturing agents can increase proteinase activity by as much as sevenfold. Working solution: Suggested Buffers: The most appropriate buffer for Proteinase K will vary from application to application. Always follow the pH and temperature guidelines in parameter filed. As a general rule, proteinase K is stable and very active in buffers that contain denaturing reagents such as urea, sodium dodecyl sulfate (SDS), and guanidinium salts. Inhibitors: The enzyme is inactivated by Pefabloc® SC. However, it is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ~2.5 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.23.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |