Aprotinin
ROCHE/ROAPRO - from bovine lung
Synonym: pancreatic trypsin inhibitor; trypsin inhibitor, pancreas type (bpti); trypsin-kallikrein inhibitor
Product Type: Chemical
| absorption | 0.84 at 280 nm |
| biological source | bovine lung |
| form | lyophilized |
| manufacturer/tradename | Roche |
| packaging | pkg of 10 mg (10236624001) |
| pkg of 100 mg (11583794001) | |
| pkg of 50 mg (10981532001) | |
| pH range | 3-10 |
| Quality Level | 100  |
| shipped in | wet ice |
| solubility | water: soluble 10 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| tissue culture: suitable |
| Application: | Aprotinin is used for the protection of proteins and enzymes during isolation/purification. The inhibition of protease activity increases the lifetime of cells in cell and tissue culture studies. • Further applications: Purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin • Quantification of kallikrein activity in mixtures of esterases and proteases • Controlled degradation of substrates by avoiding nonspecific proteolysis in clinical chemical tests • Aprotinin as a model protein in protein-folding studies • Molecular weight marker in SDS-polyacrylamide gel electrophoresis |
| General description: | Trypsin inhibitor, pancreas type from bovine lung. It is known as Pancreatic trypsin inhibitor (BPTI). Aprotinin, also known as pancreatic trypsin inhibitor and trypsin-kallikrein inhibitor, is found to be expressed in lungs, spleen, liver, and pancreas. It is also found to be present in the free form in calf serum. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 0.06 to 2 μg/ml (0.01 - 0.3 μM) Working solution: Soluble in water (10 mg/ml) or aqueous buffer solution (e.g., 0.1 M Tris, pH 8.0). Note: To avoid adsorption of aprotinin onto negatively charged solid phases, e.g., chromatography gels, ultrafiltration membranes, the NaCl concentration should be above 0.1 M or other suitable salts should be added to all buffers used during the separation. Storage conditions (working solution): -15 to -25 °C |
| Reconstitution: | Freely soluble in water (10 mg/ml) or aqueous buffer solution (e.g., Tris, 0.1 M, pH 8.0). A solution adjusted to pH 7 to 8 is stable for approximately 1 week at 2 to 8 °C. Aliquots stored at -15 to -25 °C are stable for approximately 6 months. Note: Avoid repeated freezing and thawing and exposure to strongly alkaline solutions (inactive at pH > 12.8). |
| Sequence: | Monomeric peptide of 58 amino acids held in conformation by three disulfide bonds. |
| Specificity: | Aprotinin inhibits serine proteases. It inhibits kallikrein, the protease that releases hypotensive peptides such as kallidin and bradykinin (human plasma kallikrein: Ki = 3 ×10-8 M at pH 8.0, porcine pancreas kallikrein: Ki = 1 × 10-9 M at pH 8.0), trypsin (Ki = 2.8 × 10-11 M at pH 7.8, Ki = 2.6 × 10-9 M at pH 4.0, non-competetive), trypsinogen, chymotrypsin (Ki = 9 × 10-9 M at pH 8.0), bacterial fibrinolysin, and plasmin (Ki = 1 nM at pH 7.3). Cathepsin G, acrosin, human leukocyte elastase, and human urokinase are weakly inhibited. Factor Xa, thrombin, subtilisin, papain, pepsin, angiotensin-converting enzyme (ACE), carboxypeptidase A and B, other metalloproteases, and thiolproteases are not inhibited. |
| Unit Definition: | One inhibitor unit (IU) is defined as the amount of aprotinin that completely inhibits 1 U trypsin in < 10 minutes at pH 6. (Trypsin activity determined at +25 °C, pH 8.0, BAEE as substrate). One inhibitor unit (IU) (+25 °C, BAEE as substrate) corresponds to about 2.8 inhibitor units (+25 °C, Chromozym TRY as substrate). One inhibitor unit (IU) (+25 °C, BAEE as substrate) corresponds to about 26 kallikrein inhibitor units (KIU) (+25 °C). One inhibitor unit (IU) (+25 °C, BAEE as substrate) corresponds to about 0.067 inhibitor units (+25 °C; Bz-D,L-Arg-4-Na as substrate, trypsin determination at pH 7.8). One kallikrein inhibitor unit = 0.17 μg crystalline aprotinin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |