Anti-HA (12CA5)
ROCHE/ROAHA - from mouse IgG2bκ
Synonym: antibody
Product Type: Chemical
Immunopreciptation of an HA-tagged protein with Anti-HA. HA-tagged green fluorescent protein (GFP) was immunoprecipitated by incubation with the indicated amounts (in μg) of Anti-HA, followed by incubation with Protein-G-agarose. Pellets were solubilized, electrophoresed, and western blotted. Detection was achieved using Anti-HA-Peroxidase and the BM Chemiluminescence Western Blotting Substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| assay | >90% (HPLC and SDS-PAGE) |
| biological source | mouse |
| clone | 12CA5, monoclonal |
| concentration | 5 mg/ml (11666606001) |
| conjugate | unconjugated |
| epitope sequence | YPYDVPDYA |
| form | frozen liquid (11666606001) |
| lyophilized (11583816001) | |
| isotype | IgG2bκ |
| manufacturer/tradename | Roche |
| packaging | pkg of 200 μg (11583816001) |
| pkg of 5 mg (11666606001 [1 ml]) | |
| Quality Level | 100  |
| storage temp. | −20°C |
| Analysis Note: | Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard. |
| Application: | Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using: • Dot blots • Immunochemistry • Immunoprecipitation • Western blotting Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10). |
| Biochem/physiol Actions: | Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| Biochem/physiol Actions: | Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection. |
| General description: | Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome. |
| Immunogen: | Amino acids 98-106 from the human influenza virus hemagglutinin protein. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | 11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 0.1 to 1 μg/ml • Immunfluorescence: 1-10 μg/ml • Western blot: 0.1 to 1 μg/ml |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >90% (HPLC and SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |