Protein A Agarose
ROCHE/PROTAA-RO - >98% (HPLC and SDS-PAGE), suspension
Synonym: agarose; protein a
Product Type: Chemical
Protein A Agarose
| assay | >98% (HPLC and SDS-PAGE) |
| capacity | >18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0) |
| form | suspension |
| manufacturer/tradename | Roche |
| packaging | pkg of 15 mL (05015979001 [settled resin volume]) |
| pkg of 2 mL (11719408001 [settled resin volume]) | |
| pkg of 5 mL (11134515001 [settled resin volume]) | |
| storage temp. | 2-8°C |
| Analysis Note: | Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins. |
| Application: | Purification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts and for the immunoprecipitation or co-immunoprecipitation of proteins. It has also been used for photoactivatable-ribonucl |
| Biochem/physiol Actions: | Protein A is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein A binds to varying degrees IgM, IgA, IgD, and most subclasses of IgG. |
| General description: | The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition. Protein A leakage: <18 ng Protein A/ml (ELISA) Regeneration: the gel can be used approximately 30 times Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel Protein A, immobilized. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | Preswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile. Protein A content: 3 mg/ml pre-swollen gel |
| Symbol |   GHS02,GHS07 |
| Signal word | Warning |
| Hazard statements | H226 - H319 |
| Precautionary statements | P210 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 - P403 + P235 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 102.2 °F |
| Flash Point(C) | 39 °C |
| Purity | >98% (HPLC and SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 41116133 |