Polynucleotide Kinase
ROCHE/PNK-RO - from Escherichia coli E. D. pFDX
Synonym: kinase, polynucleotide
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| optimum pH | 7.6 |
| packaging | pkg of 1,000 U (10633542001) |
| pkg of 200 U (10174645001) | |
| Quality Level | 100  |
| specific activity | >40 units/mg protein |
| storage temp. | −20°C |
| Application: | Use this polynucleotide kinase to: • Phosphorylate 5′ ends of DNA or RNA • Label 5′-hydroxyl ends of DNA or RNA with [γ-32P]-ATP (either by direct phosphorylation or by phosphate exchange) • Remove 3′-phosphates from RNA or DNA without altering their 5′ ends (at low pH only) |
| Features and Benefits: | Contents Solution, containing 10U/μl of enzyme, in Storage buffer: 50mM Tris-HCl, 1mM dithiothreitol, 0.1mM EDTA, 0.1μM ATP, 50% glycerol (v/v), pH approx. 7.5 (+25°C). Phosphorylation buffer 10x concentrated: 10x Incubation buffer for direct phosphorylation, supplied with the enzyme: 500mM Tris-HCl, 100mM MgCl2, 0.1mM EDTA, 50mM dithioerythritol, 1mM spermidine, pH 8.2 (at +25°C). |
| General description: | Polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5′-hydroxyl terminus of DNA or RNA. It also can catalyze the exchange of 5′-terminal phosphate groups. In addition, the enzyme has a 3′-phosphatase activity (only at low pH). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Polymines (spermine, spermidine) |
| Quality: | Absence of endonucleases, nicking activity, exonucleases, and ribonucleases tested according to the current Quality Control procedures. Function tested by DNA 5′-end labeling. |
| Unit Definition: | One unit is the enzyme activity which catalyzes the incorporation of 1 nmol 32P into acid-precipitable products within 30 minutes at +37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: >40 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.78 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |
| Components | Enzyme Solution, in buffer, pH 7.5 (+25 °C) 10 U/μl; Phosphorylation Buffer 10x concentrated |