Phosphoglucose Isomerase (PGI)
ROCHE/PGI-RO - from yeast
Synonym: glucose-6-phosphate isomerase
Product Type: Chemical
| biological source | yeast |
| form | suspension |
| manufacturer/tradename | Roche |
| optimum pH | 7.0-7.6 |
| packaging | pkg of 1 mL (10127396001 [2 mg]) |
| pkg of 1 mL (10128139001 [10 mg]) | |
| Quality Level | 100  |
| specific activity | ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.) |
| storage temp. | 2-8°C |
| Analysis Note: | Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase |
| Application: | Isomerization of ketoses to aldoses. |
| Biochem/physiol Actions: | Phosphoglucose isomerase (PGI) is specific for G6P (Km = 0.7 mM) and fructose-6-phosphate (F6P). |
| General description: | Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6 |
| Preparation Note: | Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 5.3.1.9 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |