Leupeptin

ROCHE/LEU-RO - powder, from synthetic, =96.5%

Synonym: Leupeptin hemisulfate salt; Acetyl-Leu-Leu-Arg-al; N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt

CAS Number: 103476-89-7
Empirical Formula (Hill Notation): C₂₀H₃₈N₆O₄ · 0.5H₂O₄S
Molecular Weight: 475.59
MDL Number: MFCD00037012
Linear Formula: C₂₀H₃₈N₆O₄ · 1/2H₂SO₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-11017101001	5 mg	$95.90 1/EA	Add To Favorites
45-11017128001	25 mg	$275.00 1/EA	Add To Favorites
45-11034626001	50 mg	$478.00 1/EA	Add To Favorites
45-11529048001	100 mg	$801.00 1/EA	Add To Favorites

Leupeptin is a modified tripeptide. HPLC analysis of leupeptin will show multiple peaks due to tautomeric isomers that exist in solution.

Properties
Descriptions
Safety Info.
Basic Data

assay	96.5%
biological source	synthetic
description	Ac-Leu-Leu-argininal x 1/2 H₂SO₄, synthetic
form	powder
InChI	1S/2C20H38N6O4.H2O4S/c21-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h211-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
InChI key	CIPMKIHUGVGQTG-VFFZMTJFSA-N
manufacturer/tradename	Roche
mol wt	M_r 475.6
packaging	pkg of 100 mg (11529048001)
	pkg of 25 mg (11017128001)
	pkg of 5 mg (11017101001)
	pkg of 50 mg (11034626001)
Quality Level	100
shipped in	wet ice
SMILES string	OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
solubility	H₂O: 50 mg/mL
storage temp.	2-8°C

Analysis Note:	Function test: Performance controlled with trypsin.
Analysis Note:	Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.
Application:	Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis. *Note:* To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na₂, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.
Biochem/physiol Actions:	Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM.
Biochem/physiol Actions:	Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)
Biochem/physiol Actions:	Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B. Not affected are α-, β-, γ- and δ- chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.
Features and Benefits:	Contents Synthetic, white powder
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Working concentration: 0.5 to 5 μg/ml (1 to 20 μM) Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files. Working solution: Solvent is recommended in distilled water. Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide. Poorly soluble in acetone, chloroform, ethyl ether and n-hexane. Storage conditions (working solution): -15 to -25 °C In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.
Preparation Note:	Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.

Symbol	GHS07
Signal word	Warning
Hazard statements	H302 + H312 + H332
Precautionary statements	P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	96.5%
Storage Temp.	2-8°C
UNSPSC	12352202

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