Klenow Enzyme
ROCHE/KLEN-RO - Labeling grade, from Escherichia coli lysogenic NM 964
Synonym: Klenow
Product Type: Chemical
| biological source | Escherichia coli |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 U (11008404001) |
| pkg of 500 U (11008412001) | |
| Quality Level | 100  |
| specific activity | >5000 units/mg protein |
| storage temp. | −20°C |
| Application: | Use Klenow Enzyme for: • Random-primed DNA labeling using random oligonucleotides as primers for the incorporation of nonradioactively labeled and 32P-labeled nucleotides. • Fill-in reaction for blunt-end formation of 3′-recessed (staggered) ends. |
| General description: | Klenow enzyme is a DNA-dependent 5′→3′ polymerase with 3′→5′ exonuclease activity. It lacks the 5′→3′ exonuclease activity of the native enzyme. The addition of mononucleotides from dNTPs to the 3′-OH terminus of DNA is catalyzed. This property is used to synthesize DNA complementary to single-stranded DNA templates. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Enzyme in Storage buffer: 50mM potassium phosphate, 1mM dithioerythritol, 50% glycerol (v/v), pH 7.0 (+4°C). |
| Quality: | Absence of endo- and exonuclease activity tested according to the current Quality Control procedures |
| Specificity: | Heat inactivation: Add 2 μl 0.2 mM EDTA (pH 8.0) and/or heat to 65 °C for 10 minutes |
| Unit Definition: | One unit is the enzyme activity which incorporates 10 nmol of total nucleotides into an acid-precipitable fraction in 30 minutes under assay conditions at +37 °C with poly [d(A-T)] as primer (Richardson, C.C. & Kornberg, A. (1994) J. Biol. Chem. 244, 2996). Volume Activity: 2,000 U/ml |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: >5000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |