High Pure PCR Product Purification Kit
ROCHE/HPPCRPKRO - sufficient for 250 purifications (11732676001), sufficient for 50 purifications (11732668001), suitable for DNA purification
Synonym: PCR purification
Product Type: Chemical
Purified concentrated DNA Procedure
High Pure PCR Product Purification Kit vs High Pure PCR Cleanup Micro Kit
Figure 1: Comparison of PCR products purified with the High Pure PCR Product Purification Kit and those purified by phenol/chloroform extraction.
Purified PCR Product Flow Chart
| manufacturer/tradename | Roche |
| packaging | pkg of 250 purifications (11732676001) |
| pkg of 50 purifications (11732668001) | |
| Quality Level | 100  |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 250 purifications (11732676001) |
| sufficient for 50 purifications (11732668001) |
| Application: | The High Pure PCR Product Purification Kit is designed for the preparation of concentrated, purified DNA, and can be used directly for most molecular biology applications: • Labeling • Sequencing • Cloning • Restriction enzyme digests • Alkaline phosphatase treatment • Kinase reactions The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions. It can also be applied to concentrate dilute nucleic-acid solutions. Use one kit for a variety of applications. The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 μl (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR. |
| Components: | • Binding Buffer • Wash Buffer • Elution Buffer • High Pure Spin Filter Tubes (containing glass fiber fleece) • Collection Tubes |
| Features and Benefits: | • Quickly purifies multiple PCR products in <10 minutes, and purify DNA from agarose gel slices in <20 minutes. • Efficiently recover DNA fragments >100 bp in length, and concentrate dilute nucleic acid solutions. • Minimize DNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade DNA. • Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide |
| General description: | Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. Capacity: The High Pure SpinFilter Tubes hold up to 700 μl sample volume. Volume: 100 μl Typical samples include: • Products from amplification or cDNA synthesis reactions • Enzymatically treated DNA • DNA from agarose slices • Dilute nucleic acid solutions • RNA from transcription reactions |
| General description: | The High Pure PCR Product Purification Kit efficiently and conveniently isolates PCR products from amplification reactions and purifies nucleic acids from other modification reactions. It is also recommended for the purification of cDNA. |
| Other Notes: | High Pure Technology and Silica Adsorption Kits |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | The sample is mixed with a chaotropic salt and applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, all nucleic acids (NA) in the sample bind to the glass fleece in the High Pure tube, while contaminating substances (salts, proteins, nucleotides, mineral oil and other contaminants) do not. Brief wash-and-spin steps readily remove these contaminants. Once purified, the NA can be easily eluted in a small volume of low salt buffer. |
| Quality: | More than 70% recovery is obtained when 10 μg DNA Molecular Weight Marker VIII mixed with 16 μg bovine serum albumin are applied to the High Pure Filter Tubes. Gel electrophoresis of the DNA eluate confirms the complete removal of protein and DNA fragments smaller than 100 bp. |
| Symbol |   GHS05,GHS07,GHS05,GHS07 |
| Signal word | Danger-Danger |
| Hazard statements | H302 + H332 - H314 - H412 - H302 + H332 - H314 - H412 |
| Precautionary statements | P261 - P273 - P280 - P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Supplemental Hazard Statements | EUH032 |
| UNSPSC | 41105500 |