Glutamate-Oxaloacetate Transaminase (GOT)
ROCHE/GOT-RO - suspension, ~200 units/mg protein (At 25 °C (380 U/mg at 37 °C) with L-aspartate and 2-oxoglutarate as the substrates), optimum pH 6.5-8.5
Synonym: aspartate aminotransferase got; got
Product Type: Chemical
| description | from pig heart |
| form | suspension |
| manufacturer/tradename | Roche |
| optimum pH | 6.5-8.5 |
| packaging | pkg of 1 mL (10105546001 [2 mg]) |
| pkg of 1 mL (10105554001 [10 mg]) | |
| pkg of 2.5 mL (10737046001 [25 mg]) | |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~200 units/mg protein (At 25 °C (380 U/mg at 37 °C) with L-aspartate and 2-oxoglutarate as the substrates) |
| storage temp. | 2-8°C |
| Application: | Synthesis of unnatural L-amino acids from α-keto acids. |
| General description: | Glutamate-Oxaloacetate Transaminase (GOT) is a transaminase enzyme which catalyzes amino group of an amino acid with keto group of keto acid. |
| General description: | L-aspartate:2-oxoglutarat |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, 2.5 mM 2-oxoglutarate, 50 mM maleate, and 1 mM pyridoxal phosphate, pH approximately 6 |
| Preparation Note: | Activator: Pyridoxal phosphate, pyridoxamine phosphate |
| Unit Definition: | Unit Conversion: One unit (+25 °C, pH 7.4) ≈ 1.9 units (+37 °C, pH 7.4) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~200 units/mg protein (At 25 °C (380 U/mg at 37 °C) with L-aspartate and 2-oxoglutarate as the substrates) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.6.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |