FastStart Universal Probe Master (Rox)
ROCHE/FSUPMMRO - sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR
Synonym: qPCR
Product Type: Chemical
Figure 1: Performance of FastStart Universal Probe Master (Rox) on an ABI 7900 Instrument.
The data show a comparison of Roche FastStart Universal Probe Master (Rox) (A) and a master mix from another supplier (B). Reactions use a hydrolysis probe specific for the globin gene and a titration of human genomic DNA at concentrations of 50, 5, 0.5, and 0 ng/well. FastStart Universal Probe Master (Rox) data demonstrates earlier Ct values and consistent, sensitive results.
The data show a comparison of Roche FastStart Universal Probe Master (Rox) (A) and a master mix from another supplier (B). Reactions use a hydrolysis probe specific for the globin gene and a titration of human genomic DNA at concentrations of 50, 5, 0.5, and 0 ng/well. FastStart Universal Probe Master (Rox) data demonstrates earlier Ct values and consistent, sensitive results.
| detection method | probe-based |
| feature | dNTPs included: no |
| hotstart | |
| input | purified DNA |
| manufacturer/tradename | Roche |
| packaging | pkg of 1250 x 20 μL reactions (04913957001) |
| pkg of 250 x 20 μL reactions (04913949001) | |
| pkg of 5000 x 20 μL reactions (04914058001) | |
| Quality Level | 100  |
| technique(s) | qPCR: suitable |
| RT-qPCR: suitable | |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Application: | FastStart™ Universal Probe Master (Rox) has been used: • in TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR) reactions for the quantification of endogenous miRNAs, such as MIR376B, MIR376A and MIR181A • in reverse transcriptase (RT-PCR) to study tumor necrosis factor (TNF) expression in whole synovial tissue of undifferentiated peripheral inflammatory arthritis (UPIA) patients • for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich by quantitativePCR Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR. |
| Components: | FastStart Universal Probe Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and a reference dye. |
| Features and Benefits: | • Increase qPCR sensitivity and specificity. Produce lower cycle threshold (Ct) values. • Use the master mix with any probe-based assay. Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe. • Amplify and detect a broad range of DNA or cDNA targets. Amplify fragments up to 500 bp long, including those that are GC- or AT-rich. • Visualize amplification products on agarose gels. • Use robotic pipetting stations to set up qPCR reactions. Use a master mix that is stable at room temperature during extended reaction setup times. • Prevent false positives resulting from carryover contamination. Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions. |
| General description: | Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C). |
| General description: | Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX. FastStart™ Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. FastStart™ Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler® Instruments. |
| Legal Information: | FastStart is a trademark of Roche |
| Legal Information: | LightCycler is a registered trademark of Roche |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures |
| Quality: | Function test: Each lot is tested for performance in qPCR using three templates: a GC–rich template, an AT-rich template, and a long template (approximately 440 bp). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 41106300 |