Fibronectin (pure)
ROCHE/FIBRP-RO - from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)
Synonym: Fibronectin
MDL Number: MFCD00131062
Product Type: Chemical
| assay | >95% (SDS-PAGE) |
| biological source | human plasma |
| form | lyophilized (clear, colorless solution after reconstitution) |
| manufacturer/tradename | Roche |
| mol wt | 440 kDa |
| packaging | pkg of 1 mg (11051407001) |
| pkg of 5 mg (11080938001) | |
| Quality Level | 100  |
| sterility | non-sterile; 0.2 μm filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02751  |
| Application: | Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay. |
| General description: | Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin |
| Preparation Note: | Working concentration: 5μg/cm2 for the coating of cell culture vessels Storage conditions (working solution): -15 to -25 °C The reconstituted solution is stable at -15 to -25 °C. Note: Store the reconstituted solution in aliquots at -15 to -25 °C. Avoid repeated freezing and thawing. |
| Reconstitution: | Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate. Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance. |
| Sequence: | Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges. |
| Specificity: | Active on most mammalian cells Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >95% (SDS-PAGE); >95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |