Enterokinase
ROCHE/ENTK-RO - from calf intestine
Synonym: restriction protease enterokinase
Product Type: Chemical
| biological source | bovine (calf) intestine |
| concentration | 1:50 % (w/w) |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 150 kDa |
| optimum pH | 8 |
| packaging | pkg of 3 × 250 μg (11351311001) |
| pkg of 3 × 30 μg (11334115001) | |
| storage temp. | 2-8°C |
| Application: | Quality Purity: highly purified, not standardized with albumin Quality control: function tested |
| Application: | Enterokinase is used for the cleavage of fusion proteins at definite cleavage sites. For the processing of recombinant proteins, the desired protein is fused with Enterokinase recognition sequence. After purification of the entire fusion protein, the protein or peptide is released by incubation with enterokinase. |
| General description: | Enterokinase is a protease from calf intestine and supplied in a quality optimized for the cleavage of fusion proteins. Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 1:50 (w/w) Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week. |
| Specificity: | Serine protease acting as a restriction protease that recognizes the amino acid sequence -(Asp)4-Lys-X. The aspartic acid residues can be partially substituted by glutamic acid. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 - P342 + P311 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.9  ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |