Endoglycosidase H
ROCHE/ENDOH-RO - recombinant form of the gene from Streptomyces plicatus
Synonym: Endo-β-N-acetylglucosaminidase H; glycosidase
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | Streptomyces sp. (S. plicatus) |
| conjugate | (N-linked) |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | Mr 29 kDa |
| optimum pH | 5-6 |
| packaging | pkg of 200 μL (11088726001 [1U]) |
| pkg of 500 μL (11643053001 [2.5 U]) | |
| Quality Level | 100  |
| specific activity | ~40 units/mg protein (at +37°C and pH 5.5 with dabsyl-Asn(GlcNAc)2(Man)5 as the substrate) |
| storage temp. | 2-8°C |
| Application: | Use Endoglycosidase H for the deglycosylation of glycoproteins. Endo H has been used for the analysis of glycosylation of ATP-binding cassette proteins. |
| General description: | Endo-β-N-acetylglucosamin Endoglycosidase H hydrolyzes N-linked oligosaccarides of glycopeptides/proteins. It cleaves only high mannose structures. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution in 50 mM sodium phosphate, 25 mM EDTA, 0.1% Micr-O-protect; (w/v), pH 7 |
| Quality: | Absence of contaminants: α- and β-glucosidase, β-galactosidase, α-mannosidase, β-N-acetyl-hexosaminidase, α-L-fucosidase, and proteases are not detectable. |
| Specificity: | Active on N-linked oligosaccharides of glycopeptides/proteins. Cleaves only high-mannose structures (n = 2 - 150, x = [Man]1-2, y = H), and hybrid structures (n = 2, x and/or y = AcNeu-Gal-GlcNAc). |
| Unit Definition: | One unit enzyme activity hydrolyzes 1 μmol dabsyl-Asn(GlcNAc)2(Man)5 or 0.2 μmol dansyl-Asn (GlcNAc)2(Man)5 in one minute at 37 °C and pH 5.5. Note: 5 U, using the dabsyl substrate, corresponds to 1 U using the dansyl substrate. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ~40 units/mg protein (at +37°C and pH 5.5 with dabsyl-Asn(GlcNAc)2(Man)5 as the substrate) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.96 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |