Expand™ High FidelityPLUS PCR System
ROCHE/EHIFIP-RO - sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no
Synonym: high fidelity pcr plus system, expand
Product Type: Chemical
Comparison of Expand High FidelityPLUS PCR System to four commercially available PCR mixes. Human genomic DNA was used to amplify a 4.8 kb tPS gene fragment, using each supplier’s instructions.
Expand high fidelity plus PCR System
| feature | dNTPs included: no |
| High Fidelity PCR | |
| hotstart: no | |
| input | purified DNA |
| manufacturer/tradename | Roche |
| packaging | pkg of 2,500 U (03300234001 [10x250 U]) |
| pkg of 500 U (03300226001 [2x250 U]) | |
| parameter | 72 °C optimum reaction temp. |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for ≤2,500 reactions (03300234001) |
| sufficient for ≤500 reactions (03300226001) | |
| sufficient for 2500 reactions | |
| sufficient for 500 reactions |
| Application: | The Expand™ High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay. Use Expand High FidelityPLUS PCR System for: • PCR • RT-PCR • Primer extension • Carryover prevention |
| Features and Benefits: | • Safeguard against cross-contamination. This is the choice for large numbers of amplifications. • Obtain sixfold higher fidelity and higher yields, compared to Taq DNA Polymerase. • Efficiently label DNA. Use this kit to label DNA with radioactive or nonradioactive nucleotides. |
| General description: | The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity. The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone. Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets. |
| Legal Information: | Expand is a trademark of Roche |
| Legal Information: | Transition-metal-catalyze |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 4 components |
| Quality: | Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product. |
| Unit Definition: | Volume Activity: 5 U/μL |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 41106300 |
| Components | Expand High FidelityPLUS Enzyme Blend 5 U/μl; Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated; Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated; MgCl2 Stock Solution 25 mM |