EagleTaq Universal Master Mix (ROX)
ROCHE/EAGLETMMRO - sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR
Synonym: Taq | PCR | Polymerase | RT-PCR
Product Type: Chemical
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| input | purified DNA |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 x 20 μL reactions (07260288190) |
| pkg of 500 x 20 μL reactions (07260296190) | |
| pkg of 5000 x 20 μL reactions (07249926190) | |
| Quality Level | 100  |
| technique(s) | qPCR: suitable |
| RT-qPCR: suitable | |
| usage | sufficient for 100 reactions |
| sufficient for 500 reactions | |
| sufficient for 5000 reactions |
| Application: | EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR). |
| Components: | Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX. |
| Features and Benefits: | • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis. • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination. • The hot start properties allow reaction setup at ambient temperature. • With this robust reagent, any PCR protocol optimization is minimized. |
| General description: | 2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments. The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis. The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination. The hot start properties allow reaction setup at ambient temperature. With this robust reagent, any PCR protocol optimization is minimized |
| General description: | The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 41106300 |