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dTTP

ROCHE/DTTP-RO - PCR Grade, sodium salt

Synonym: Thymidine 5′-triphosphate sodium salt solution; TTP

CAS Number: 18423-43-3
MDL Number: MFCD00080338
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11934546001 250 µL
$81.20
1/EA
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assay 99% (dNTP)
concentration <0.9% (dNDP)
  100 mM
form solution
InChI 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key KAELIXTVSANDSE-UHFFFAOYSA-N
manufacturer/tradename Roche
packaging pkg of 1,250 μL (11969048001 [100 mM])
  pkg of 250 μL (11934546001 [100 mM])
  pkg of 4 × 1,250 μL (03732711001 [4x100 mM])
Quality Level 100 
SMILES string [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
storage temp. −20°C
usage sufficient for 125,000 standard reactions (03732711001)
  sufficient for 31,250 standard reactions (11969048001)
  sufficient for 6,250 standard reactions (11934546001)
Analysis Note: Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Application: dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.
General description: dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).
General description: PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
General description: Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
Other Notes: For general laboratory use.
Physical form: solution of dTTP in water (pH 8.3).
Preparation Note: Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity 99% (dNTP)
Storage Temp. −20°C
UNSPSC 12352302

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