DTT
ROCHE/DTT-RO - crystalline powder, =97% (Ellman′s reagent), Mr 154.3
Synonym: DL-Dithiothreitol; 1,4-dithiothreitol; cleland′s reagent; dithiothreitol, 1,4-; threo-
CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C4H10O2S2
Molecular Weight: 154.25
MDL Number: MFCD00004877
Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
Product Type: Chemical
| assay | =97% (Ellman′s reagent) |
| description | 1,4-Dithiothreitol |
| form | crystalline powder |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6 |
| InChI key | VHJLVAABSRFDPM-QWWZWVQMSA |
| manufacturer/tradename | Roche |
| mol wt | Mr 154.3 |
| mp | 41-44 °C (lit.) |
| packaging | pkg of 10 g (10708984001) |
| pkg of 2 g (10197777001) | |
| pkg of 25 g (11583786001) | |
| reaction suitability | reagent type: reductant |
| SMILES string | O[C@H](CS)[C@H](O)CS |
| storage temp. | 2-8°C |
| Application: | 1,4-dithiothreitol is used in: • Isolation, purification and characterization of proteins and enzymes • Measurement of enzyme activities (reactivation of enzymes) • Determination of disulfide groups in proteins and enzymes • DNA extraction prior to amplification |
| Application: | An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystami |
| General description: | 1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen. |
| Sequence: | Enantiomers Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT. |
| Specifications: | Oxidized form: <2.5% (absorbance at 283nm) |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 |
| Precautionary statements | P264 - P270 - P280 - P301 + P312 + P330 - P305 + P351 + P338 + P310 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Purity | =97% (Ellman′s reagent) |
| mp | 41-44 °C (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |