Deoxynucleoside Triphosphate Set
ROCHE/DNTP-RO - PCR Grade, sodium salt
Synonym: datp; dctp; dgtp; dttp; set of datp, dctp, dgtp, and dttp, pcr grade
Product Type: Chemical
| assay | <0.9% (dNDP) |
| 99% (dNTP) | |
| manufacturer/tradename | Roche |
| packaging | pkg of 4 × 1,250 μL (03622614001 [4x100 mM]) |
| pkg of 4 × 250 μL (11969064001 [4x100 mM]) | |
| Quality Level | 100  |
| storage temp. | −20°C |
| usage | sufficient for 37,500 reactions (03622614001) |
| sufficient for 6,250 reactions (11969064001) |
| Application: | The Deoxynucleoside Triphosphate Set, PCR Grade, is suitable for many applications where high-quality reagents are required. Such applications include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis. |
| Features and Benefits: | PCR Grade Nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. • PCR Grade Nucleotides contain at least 99% of the relevant deoxynucleoside-triphosph • They are provided as clear, colorless solutions in water at a concentration of 100 mM (pH 8.3). • Due to the manufacturing process, PCR Grade Nucleotides are free of dNTPs with modified bases, as well as tetra- and pyrophosphate contamination. Tetraphosphates can disintegrate and form pyrophosphate, which is a known inhibitor of amplification reactions. Therefore, the purity of nucleotides is critical to the success of your amplification reactions. • PCR Grade Nucleotides are stable for 30 months after the production date when stored at -15 to -25°C. |
| General description: | This product contains 4 separate vials of dATP, PCR Grade, dCTP, PCR Grade, dGTP, PCR Grade, and dTTP, PCR Grade, each with a concentration of 100 mM and as a clear, colorless solution of the sodium salt (pH 8.3). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 set containing 4 components • dATP, PCR Grade, sodium salt, 100 mM • dCTP, PCR Grade, sodium salt, 100 mM • dGTP, PCR Grade, sodium salt, 100 mM • dTTP, PCR Grade, sodium salt, 100 mM |
| Preparation Note: | Working solution: For best results, prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade). |
| Quality: | Function tested using RT-PCR: Tested for absence of contaminating deoxyribonucleases, ribonucleases, and nicking activities according to the current Quality Control procedures. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | <0.9% (dNDP); 99% (dNTP) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | dATP, PCR Grade, sodium salt 100 mM; dCTP, PCR Grade, sodium salt 100 mM; dGTP, PCR Grade, sodium salt 100 mM; dTTP, PCR Grade, sodium salt 100 mM |