DNA Polymerase I
ROCHE/DNAPOL-RO - from Escherichia coli lysogenic for NM 964
Synonym: kornberg polymerase
Product Type: Chemical
| biological source | Escherichia coli |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 1,000 U (10642720001) |
| pkg of 250 U (10642711001) | |
| Quality Level | 100  |
| shipped in | dry ice |
| specific activity | 8000 units/mg protein (At least 8,000 U/mg, using poly [d(A-T)] as template at 37 °C, according to Richardson (Richardson, C. C. et al., 1964, J. Biol. Chem. 239, 222).) |
| storage temp. | −20°C |
| General description: | Recombinant DNA polymerase I is a DNA-dependent 5′→ 3′ polymerase that has both 5′ → 3′ and 3′ → 5′ exonuclease activities. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, 5 U/μl in storage buffer: 50 mM potassium phosphate, 0.25 mM dithiothreitol, 50%glycerol (v/v); pH approx. 7. |
| Preparation Note: | Activator: Mg2+ |
| Quality: | Tested for the absence of endonucleases according to the current Quality Control procedures. |
| Specificity: | Specific Activity: 8,000 U/mg At least 8,000 U/mg, using poly [d(A-T)] as template at +37°C, according to Richardson. Source: The enzyme is the product of the E. coli pol A gene, which has been cloned into an overproducing lysogenic strain. |
| Unit Definition: | One unit is the enzyme activity which incorporates 10 nmol of total nucleotide into an acid-precipitable fraction in 30 minutes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 8000 units/mg protein (At least 8,000 U/mg, using poly [d(A-T)] as template at 37 °C, according to Richardson (Richardson, C. C. et al., 1964, J. Biol. Chem. 239, 222).) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |