Digoxigenin-11-dUTP, alkali-stable
ROCHE/DIUTPS-RO
Synonym: digoxigenin
Product Type: Chemical
| λmax | 222 and 292 nm |
| 22430 and 8780 | |
| assay | ≥85% (HPLC) |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | Mr 1090.7 |
| packaging | pkg of 125 μL (11558706910 [1 mM]) |
| pkg of 25 μL (11093088910 [1 mM]) | |
| pkg of 5 × 125 μL (11570013910 [5x1 mM]) | |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | Digoxigenin-11-dUTP, alkali-stable has been used to label probes for fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR) Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP. |
| Biochem/physiol Actions: | Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase. |
| Features and Benefits: | Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage. Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C. |
| General description: | Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade). Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C. |
| Quality: | Typical analysis: At least 85% DIG-11-dUTP (HPLC, area%). Function tested by RPL. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥85% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |