Digoxigenin-11-UTP
ROCHE/DIGUTP-RO
Product Type: Chemical
| assay | ≥85% (HPLC) |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | (Mr = 1106.7 (DIG-11-UTP-Li4)) |
| packaging | pkg of 25 μL (11209256910 [10 mM]) |
| pkg of 57 μL (03359247910 [3.5 mM]) | |
| Quality Level | 100  |
| storage temp. | −20°C |
| Analysis Note: | Typical analysis: 85% DIG-11-UTP (HPLC, area%) Function tested with the DIG RNA Labeling Kit. Formula: C43H61N4O22P3Li4 |
| Application: | Digoxigenin-11-UTP has been used for labeling RNA probes in: • in situ hybridization • in situ reverse transcriptase (RT)-polymerase chain reaction (PCR) • fluorescence in situ hybridization (FISH) |
| Biochem/physiol Actions: | Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids. |
| General description: | Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P264 - P270 - P301 + P312 + P330 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥85% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |