dATP
ROCHE/DATP-RO - 99% (dNTP), <0.9% (dNDP), solution, 100 mM
Synonym: 2′-Deoxyadenosine 5′-triphosphate sodium salt solution; dATP
Product Type: Chemical
| assay | <0.9% (dNDP) |
| 99% (dNTP) | |
| concentration | 100 mM |
| description | PCR Grade, sodium salt |
| form | solution |
| InChI | 1S/C10H16N5O12P3.Na/c11-9 |
| InChI key | YJWCICGGRLOGEH-VWZUFWLJSA |
| manufacturer/tradename | Roche |
| packaging | pkg of 1,250 μL (11969013001 [100 mM]) |
| pkg of 250 μL (11934511001 [100 mM]) | |
| pkg of 4 × 1,250 μL (03732681001 [4x100 mM]) | |
| Quality Level | 100  |
| SMILES string | [Na+].Nc1ncnc2n(cnc12)[C@ |
| storage temp. | −20°C |
| usage | sufficient for 125,000 standard reactions (03732681001) |
| sufficient for 31,250 standard reactions (11969013001) | |
| sufficient for 6,250 standard reactions (11934511001) |
| Application: | 2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques. |
| Application: | Quality Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures. |
| Application: | dATP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis. |
| General description: | 2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5’-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases. |
| General description: | dATP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosph |
| Other Notes: | For general laboratory use. |
| Physical form: | solution of dATP in water (pH 8.3). |
| Preparation Note: | Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | <0.9% (dNDP); 99% (dNTP) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |